Publication
535
J. Am.
Chem. Soc., 123
(20), 4817 -4825, 2001
DOI: 10.1021/ja004050f S0002-7863(00)04050-6 |
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Elastic Bounded Diffusion and Electron Propagation: Dynamics of
the Wiring of a Self-Assembly of Immunoglobulins Bearing
Terminally Attached Ferrocene Poly(ethylene glycol) Chains
According to a Spatially Controlled Organization
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Agnès Anne,
Christophe Demaille, and Jacques Moiroux
Contribution from the Laboratoire d'Electrochimie Moléculaire, Unité Mixte de Recherche Université -
CNRS No 7591, Université de Paris 7 - Denis Diderot, 2 place Jussieu, 75251 Paris Cedex 05, France
Molecular monolayers of immunoglobulins bearing terminally attached ferrocene poly(ethylene glycol)
chains (IgG-PEG-Fc) were self-assembled at an electrode surface in a step-by-step manner involving antigen-antibody recognition reactions. The total number N of assembled IgG-PEG-Fc monolayers and the number
of spacers ni separating two successive IgG-PEG-Fc monolayers were controlled and varied. Electron transport
through the protein assembly involves the dynamics of the terminally attached PEG chains and isotopic electron
exchange between ferrocene heads belonging to successive IgG-PEG-Fc monolayers. The model of elastic
bounded diffusion enabled us to analyze quantitatively the dependence of the rate of electron transport on N,
ni, and the rate constant (ke) of isotopic electron exchange. Wiring of a molecular monolayer of redox enzyme
is also quantitatively characterized. |
