Publication
540
Analyst, 126 (6), 887-891, 2001
DOI: 10.1039/b100533m |
|
|
Determination of horseradish peroxidase and
a peroxidase-like iron porphyrin at a Nafion-modified
electrode
|
Chantal Degrand, Benoît Limoges,
Anne-Marie Martre and
Bernd Schöllhorn
Contribution from the Laboratoire d'Electrochimie Moléculaire, Unité Mixte de Recherche Université -
CNRS No 7591, Université de Paris 7 - Denis Diderot, 2 place Jussieu, 75251 Paris Cedex 05, France
A catalytic coupling reaction between 4-amino antipyrine and a
N,N-disubstituted aniline derivative has been exploited in
the indirect electrochemical detection of horseradish peroxidase
(HRP) and of a biomimetic catalyst, the iron(iii)
sulfonated tetraphenyl porphyrin. In the presence of hydrogen
peroxide and one of the two catalysts a cationic electroactive
quinone-iminium dye P+ was formed and detected by linear
scan voltammetry using a screen-printed electrode coated with a
Nafion film. Detection limits of 10-12 M for HRP and 4 ×
10-10 M for the iron porphyrin have been achieved. In
conclusion the iron porphyrin is considered to be a promising
alternative to the HRP label in enzyme immunoassays with
electrochemical detection. |
