Publication
541
Anal.
Chem., 73 (8), 4450-4456, 2001
DOI: 10.1021/ac0103221 S0003-2700(01)00322-5 |
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Gold Nanoparticle-Based Quantitative Electrochemical
Detection of Amplified Human Cytomegalovirus DNA Using Disposable Microband
Electrodes
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Laurent Authier, Céline Grossiord, Pierre Brossier and Benoît Limoges
Contribution from the Laboratoire de Microbiologie Médicale et Moléculaire, Faculté de Médecine
et de Pharmacie, 7 Boulevard Jeanne d'Arc, 21033 Dijon, France and the Laboratoire d'Electrochimie Moléculaire, Unité Mixte de Recherche Université -
CNRS No 7591, Université de Paris 7 - Denis Diderot, 2 place Jussieu, 75251 Paris Cedex 05, France
An electrochemical DNA detection method has been developed for the sensitive
quantification of an amplified 406-base pair human cytomegalovirus DNA sequence
(HCMV DNA). The assay relies on (i) the hybridization of the single-stranded
target HCMV DNA with an oligonucleotide-modified Au nanoparticle probe, (ii)
followed by the release of the gold metal atoms anchored on the hybrids by
oxidative metal dissolution, and (iii) the indirect determination of the
solubilized AuIII ions by anodic stripping voltammetry at a
sandwich-type screen-printed microband electrode (SPMBE). Due to the enhancement
of the AuIII mass transfer by nonlinear diffusion during the
electrodeposition time, the SPMBE allows the sensitive determination of
AuIII in a small volume of quiescent solution. The combination of the
sensitive AuIII determination at a SPMBE with the large number of
AuIII released from each gold nanoparticle probe allows detection of
as low as 5 pM amplified HCMV DNA fragment. |