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Laboratoire d'Electrochimie Moleculaire, LEM, Paris

UMR CNRS - Université Paris Diderot - Paris France

Master Frontiers in Chemistry | UFR de Chimie - Université Paris Diderot - Paris 7 CNRS - Institut de chimie Université de Paris Master Chimie Sorbonne Paris Cité UFR de Chimie - Université Paris Diderot - Paris 7 CNRS - Institut de chimie
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Université Paris Diderot
Université de Paris CNRS, Centre National de la Recherche Scientifique

Le LEM - Publications: Abstracts

Publication 573

J. Am. Chem. Soc., 125 (45), 13686 -13692, 2003
DOI: 10.1021/ja0363819 S0002-7863(03)06381-9


Electrocatalytic Investigation of Light-Induced Electron Transfer between Cytochrome

Vanessa Proux-Delrouyre, Christophe Demaille, Winfried Leibl, Pierre Sétif, Hervé Bottin and Christian Bourdillon

Contribution from the Service de Bioénergétique, DBJC, CEA Saclay, URA du CNRS N° 2096, 91191 Gif-sur-Yvette, France; Laboratoire d'Electrochimie Moléculaire, UMR du CNRS N° 7591, Université Paris 7 - Denis Diderot, 75251 Paris Cedex 05, France; and Laboratoire de Technologie Enzymatique, UMR du CNRS N° 6022, Université de Technologie de Compiègne, B. P. 20529, 60205 Compiègne Cedex, France. E-mail:

A light-activated electron-transfer chain was assembled using solubilized cyanobacterial photosystem I as photoactive enzyme, cytochrome c6 (also from cyanobacteria) as electron donor, and methyl viologen as electron acceptor. The photocatalytic activity of the ensemble was measured by direct and reversible electrochemistry of cytochrome c6 at a surface-modified gold electrode. Analysis of the electrochemical response with an appropriate model for the reaction mechanism allowed the relation of the overall catalytic reaction rate to the individual steps of the catalytic cycle. Second-order rate constants were determined for the first time under steady-state conditions. The results validate this approach as an efficient method for the study of electron transfer between photoactive enzymes and their redox partners.
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