Publication
600
Anal. Chem. , 78, 3138-3143, 2006.
DOI: 10.1021/ac052176v
|
|
|
Redox Enzymes Immobilized on Electrodes with Solution Cosubstrates. General Procedure for Simulation of Time-Resolved Catalytic Responses
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Claude P. Andrieux, Benoît Limoges, Damien Marchal,* and Jean-Michel Savéant*
Contribution from the Laboratoire d'Electrochimie Moléculaire, Université de Paris 7-Denis Diderot, 2 place Jussieu,75251 Paris Cedex 05, France
In view of the existing and potential applications of electrochemical
enzymatic catalysis with redox enzymes immobilized on the electrode surface in
biosensors, a numerical calculation procedure for simulating their cyclic
voltammetric responses is presented. It is applicable to systems involving a
redox cosubstrate in solution. The cosubstrates, substrates, products, and
inhibitors are assumed to diffuse linearly (planar electrode) between the
electrode and the solution. The reactions in which the various forms of the
immobilized enzyme participate may be as numerous and intricate as required by
the simulation with no other restriction than the computing time. They may, at
will, follow or not follow Michaelis-Menten kinetics. Slow charge-transfer
cosubstrates are treated in the framework of Butler-Volmer kinetic law.
SIMSENSOR is a free electrochemistry software developped by the Laboratoire d'Electrochimie Moléculaire (LEM, Paris, France) for Simulation of Cyclic Voltammetric and Single-Step Chronoamperometric Catalytic Responses for Redox Enzymes Immobilized on Electrodes with Solution Cosubstrates
|