retour à l'accueil

Laboratoire d'Electrochimie Moleculaire, LEM, Paris

UMR CNRS - Université Paris Diderot - Paris France

   
 
Master Frontiers in Chemistry | UFR de Chimie - Université Paris Diderot - Paris 7 CNRS - Institut de chimie Université de Paris Master Chimie Sorbonne Paris Cité UFR de Chimie - Université Paris Diderot - Paris 7 CNRS - Institut de chimie
 |   Ecole Doctorale 388  |    Master Frontiers in Chemistry   |   C'Nano IdF   |   Respore  |
Université Paris Diderot
Université de Paris CNRS, Centre National de la Recherche Scientifique
 
 


Le LEM - Publications: Abstracts

Publication 607

J. Phys. Chem. B , 110, 21987-21993, 2006.
DOI: 10.1021/jp062806c
 

Electrochemical Approach to the Repair of Oxetanes Mimicking DNA (6-4) Photoproducts

Fabien Boussicault and Marc Robert

Contribution from the Laboratoire d'Electrochimie Moléculaire, Université de Paris 7-Denis Diderot, 2 place Jussieu,75251 Paris Cedex 05, France

 


Electrochemical study of oxetanes mimicking DNA (6-4) photoproducts gives new insight into the repair mechanism by (6-4) photolyase. Both electrochemical oxidation and electrochemical reduction at carbon electrodes lead to the cleavage of the oxetanes in a retro-Paterno-Büchi sequence. Within the family of compounds investigated and the range of driving forces offered, transient formation of unstable radical ions is observed, for both oxidative and reductive cleavage. Taking advantage of the electrochemical signature of these mimics, enzymatic assay with Escherichia coli CPD photolyase coupled to electrochemical monitoring of the reaction brings evidence that enzymatic repair of (6-4) DNA photoproducts does involve a catalytic dissociative electron-transfer mechanism at the level of an oxetane intermediate.
 
   
 
© 2005 LEM CréditsContactVenir au LEM