Publication
635
Chem. Eur. J. 14, 7186-7192, 2008.
DOI:
10.1002/chem.200800368
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DNA Damage and Radical Reactions: Mechanistic Aspects, Formation in Cells and Repair Studies
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Véronique Balland , Christelle Hureau , Angela Maria Cusano , Yingli Liu , Thierry Tron, Benoît Limoges
Laboratoire de Thermodynamique et Interactions Moléculaires, Université Blaise Pascal, Aubière, France, and
BiosCiences, Institut Sciences Moléculaires Marseille (ISM2), UMR CNRS 6263, Aix-Marseille Université Centre Scientifique Saint Jérôme, 13397 Marseille Cedex 20, France
The formation of a dense monolayer of histidine-tagged recombinant laccase on gold electrodes by using a short thiol-NTA linker is described, as well as a kinetic analysis of the process by cyclic voltammetry. From a detailed analysis of the catalytic reduction of dioxygen by laccase in the presence of a one-electron redox mediator it can be concluded that the immobilized enzyme remains as active as in homogeneous solution. |