Publication 716

Langmuir, 28 (41),14665-14671, 2012 DOI:10.1021/la3030827
Kinetics of Enzyme Action on Surface-Attached Substrates: A Practical Guide to Progress Curve Analysis in any Kinetic Situation

Laboratoire d’Electrochimie Moléculaire, UMR 7591 CNRS, Univiversité Paris Diderot, Sorbonne Paris Cité, 15 rue Jean-Antoine de Baïf, F-75205 Paris Cedex 13, France

In the present work exact kinetic equations describing the action of an enzyme in solution on a substrate attached to a surface have been derived in the framework of Michaelis-Menten mechanism, but without resorting to the often used steady-state approximation. The here-derived kinetic equations are casted in a workable format allowing us to introduce a simple and universal procedure for the quantitative analysis of enzyme surface kinetics valid for any kinetic situation. The results presented here should allow experimentalists studying the kinetics of enzyme action on immobilized substrates to analyze their data in a perfectly rigorous way.